Research Article

Molecular Study Of MDR P. mirabilis Genes Isolates From different clinical Infections

Abstract

In this study, the patients specimens of different infections were obtained from hospitals and specialized centers in Najaf province, a total of specimens 120 (100%), which included urine specimens 60 (50%), burn swabs 15 (12.5%), wound infections 15 (12.5%), ear swabs 15 (12.5%), Blood infections 15 (12.5%). Out of 120, only 95 (79.16%) were considered positive growth and 25 (20.83%) showed non- bacterial growth. On the other hand, P. mirabilis isolates where found in different clinical cases included 55 (57.89%) isolates in urine, 16 (16.84 %) isolates in burn swabs, 9 (9.47%) isolates in wound infections, 8 (8.42%) isolates in ear swabs, 7 (7.36%) isolates from the blood, From October 2024 to January 2025. Specimens were taken from patients hospitalized in Al-Hakim General Hospital, Al-Sadr Medical City, and the Burn Center in Najaf Al-Ashraf, and they were transferred to the Microbiology Laborator in the Biology Department in the Faculty of Science. The results showed that P. mirabilis isolates has a great resistance to most commonly antibiotics used in treatment of different infections, the highest rate of resistance is seen with Tetracycline 60/60 (100%) followed by Doxycycline 59/60 (98%), Gentamycin 58/60 (96%), Nalidixic Acid 58/60 (96%), Tobramycin 54/60 (90%), Ceftazidime 54/60 (90%), Ciprofloxacin 52/60 (86%), Trimethoprime- sulfamethaxazol 52/60 (86%), Amoxicillin- clavulanic 48/60 (80%), Cefotaxime 45/60 (75%), Ceftriaxone 45/60 (75%) whereas the low rate of P. mirabilis within Impenem 6/60 (10%), Meropenem 2/60 (3%), Amikacin 0/60 (0%). The results showed that the capacity of some P. mirabilis isolates to biofilm formation, out of 60 (100%) isolates of P. mirabilis only 45 (75%) were give positive results with biofilm formation and appear black dry crystalline colonies on the CRA plates and 15 (25%) were non - biofilm producers when the colonies of P. mirabilis producer remained pink or red colored, The results showed that the sul1 gene was detected in P. mirabilis isolates, out of 26 (100%) isolates only 20 (77 %) were have sul1 gene , The results also showed that the (GyrA) gene was detected in P. mirabilis which were in 25 (96%) isolates , the results display 5 (19%) isolates were have Ant (2)-1a gene, Regarding SHV gene was detected in 8(30%) of P. mirabilis isolates, while the results showed that the rmtB gene 24 (92v%) of P. mirabilis isolates.

Author's Information:

Ahmed A. S. Arak. University of Kufa, Faculty of Science, Department of Biology, Kufa, Najaf, Iraq
Baydaa A. Hassan (ORCID). University of Kufa, Faculty of Science, Department of Biology, Kufa, Najaf, Iraq