Research Article
Molecular study Of Antibiotics Resistance MDR Klebsiella pneumoniae isolated from Different Clinical Infections
- By Noor Alhuda N. A. Majed, Baydaa A. Hassan - 25 Mar 2025
- Current Research in Interdisciplinary Studies, Volume: 4, Issue: 2, Pages: 26 - 32
- https://doi.org/10.58614/cris424
- Received: 05.01.2025; Accepted: 12.03.2025; Published: 25.03.2025
Abstract
This study was completed in laboratories of Biology Department in Faculty of Science. It explains of antibiotics resistance Klebsiella pneumoniae that isolated from different infection patients in the province of Najaf. A total number of (120) samples were collected from patients with from hospitals and laboratories AL-Najaf Governorate, K. pneumoniae isolated from different infection revealed that 60 resistance K. pneumoniae isolates against 18 commonly used antibacterial agents by using the disk diffusion method K. pneumoniae isolates has a great resistance to most commonly antibiotics used in treatment of different clinical cases, the highest rate of resistance is seen with Ticarcillin / Clavulanic acid (TCC) 55(68.75%) followed by CO-Trimoazole (COT) 48(60%), Ceftazidime (CAZ) 44 (73.3%), Amoxicillin- clavulanic (AMC) 33 (55%), The results showed that the (sul1) gene was detected in K. pneumonia isolates, from the 25 (100%) isolates of K. pneumonia 19 (76%) were have (sul1) gene, The results also showed that the (blaSHV) gene was detected in K. pneumonia isolates, from the 25 (100%) isolates of K. pneumonia 6 (24%) were have (blaSHV) gene, while the results showed 6 positive isolates of the Ant (2”)-1a, from the 25 (100%) isolates of K. pneumonia 6 (24%) were have (Ant (2”)-1a) gene, Regarding (gyrA) gene the result presented that the gyrA gene was detected in K. pneumonia isolates, from the 25 (100%) all isolates of K. pneumonia were have gyrA gene. The results also displayed that the blaVIM was detected in K. pneumonia isolates, from the 25 (100%) isolates of K. pneumonia 24 (96%) were have blaVIM gene, While The results showed that the rmtB was detected in K. pneumonia isolates, from the 25 (100%) isolates of K. pneumonia 24 (96%) were have rmtB gene.
Author's Information:
Noor Alhuda N. A. Majed. University of Kufa, Faculty of Science, Department of Biology, Kufa, Najaf, Iraq
Baydaa A. Hassan (ORCID). University of Kufa, Faculty of Science, Department of Biology, Kufa, Najaf, Iraq