Research Article

Isolation and diagnosis new strain of fungi causes gingivitis

Abstract

An inflammation of the gum tissue, gingivitis is frequently brought on by a bacterial infection. Using the ITS1 sequence pattern identification approach, this study planned to isolate and identify novel fungi from the gingival region. According to the findings of the present investigation, the fungal isolates that were identified are belonging to the genus Cystobasidium, namely the species Cystobasidium minutum, Cystobasidium terricola, and Cystobasidium slooffiae. These strains were accurately diagnosed at the the species levels, matched 96–99% of the reference sequences included within the Gen-Bank dataset. The three strains belong to Cystobasidium minutum were deposited under accession numbers PX129019, PX129020, and PX129021. Additionally, the study found polymorphic features that confirmed the presence of reducing mutations and other mutations that change amino acids. This suggests that there is intraspecific heterogeneity, which may indicate that these fungi can adapt to the oral environment in pathological sites like gingivitis. The documentation of the presence of Cystobasidium in the inflamed gingival area warrants future research into the colonization sites of this fungal genus, which has previously been documented in other environmental and clinical sources.