Research Article
Antibiotic resistance gene among Carbapenem-Resistant Gramnegative Bacteria Harbour NDM gene in Al-Najaf City, Iraq
- By Ali Nurii Mardan, Basaad Abd Zaid AL-Fatlawi, Nabil Salim Saaid Tuwaij - 13 Jun 2025
- Journal of Applied Health Sciences and Medicine, Volume: 5, Issue: 6, Pages: 13 - 20
- https://doi.org/10.58614/jahsm563
- Received: 16.02.2025; Accepted: 26.05.2025; Published: 13.06.2025
Abstract
The spread of carbapenem-resistant bacteria has increased across many species and genera. As a result, the effectiveness of carbapenem treatment has diminished over time. Therefore, the aim of this work was to study carbapenem-resistant Gram-negative bacterial isolates. The current study were revealed out of 1031 clinical samples from different sources, The distribution of total patients by age group including. The age group was (1-20 years) the number of patients was 222(21.53%), the number of females was 141(19.05%) and while the males were 81(27.83%). For the age group (21-40 years) number of patients was 264(25.60%), number of females was 188(25.40%) and the number of males was 76(26.11%). The age group of (41-60 years) was the number of patients 435(42.19%) number of females was 355(47.97%) and the number of males 80(27.49%). Over 61 the number of patient 110(10.66%), the number of females was 56(7.56%) and the number of males was 54(18.55%). The current study showed that females are more likely to be affected by drug resistance than males in the age group of (41-60). By using PCR technique to screen genes responsible for carbapenem resistance. Molecular data from this work showed revealed high rate and frequencies of NDM-3, Tem, Tet A and Sul -1 genes which reached 28/30(93.33%) respectively. While Tet B, Qnr B, Sul -2 and Sul-3 were 29/30(96.61%), 27/30(90%), 23/30(76.66%) and 5/30(16.66%) respectively.
Author's Information:
Ali Nurii Mardan. Department of Biology Faculty of Science, University of Kufa, Iraq.
Basaad Abd Zaid AL-Fatlawi. Department of Biology Faculty of Science, University of Kufa, Iraq.
Nabil Salim Saaid Tuwaij. Department of Biology Faculty of Science, University of Kufa, Iraq.
How To Cite: A.N. Mardan, B.A.Z. AL-Fatlawi, and N.S.S. Tuwaij. Antibiotic resistance gene among Carbapenem-Resistant Gram-negative Bacteria Harbour NDM gene in Al-Najaf City, Iraq. Journal of Applied Health Sciences and Medicine, 5(6):13-20, 2025. https://doi.org/10.58614/jahsm563