Research Article

Genetic Analysis in Exon2 of TP53 Gene Among Colorectal Cancer Patients in Najaf City

Abstract

Colorectal cancer (CRC), which is also known as cancer of the rectum or colon, is a significant contributor to cancer-related mortality on a global scale. Age, having polyps, having inflammatory bowel disease, lifestyle, having certain genes, and having a family medical history are all things that have been shown to put people at risk for CRC. The significance of the present study is in examining the mutations occurring in exon 2 of the TP53 gene, which acts as a tumor suppressor gene. The study aims to determine the presence of mutations that are linked to colorectal cancer and their impact on the effectiveness of chemotherapy treatment.
As well as to know the relationship between diagnosed mutations and between each (health status - tumor size – stage). Blood samples were collected from 25 patients with breast cancer from the collected by the Laboratory of Center of Oncology in AL-Najaf as they were compared with 10 who were apparently healthy. The diagnosis of mutation in both patients and healthy subjects has been studied and compared to see the effect of mutations on any of them.
As a conclusion the study showed that all eight mutations in exon2 of the TP53 gene in colon cancer could be diagnosed. DNA from blood samples was taken out several times, and the mutations were found by sequencing the DNA after it was amplified by PCR technology .The single nucleotide polymorphism (SNP) rs, identified in patients with colorectal cancer, has potential as a reliable predictive tool or genetic prediction test for colorectal cancer, particularly if it yields positive results in a larger sample size.