Research Article

Estimation Activity of Chitosan Nanoparticles Biosynthesized Locally by Bacillus subtilis against Bacterial Pneumococci Activity

Abstract

Bacillus subtilis used as a biogenic bacterium to biosynthesized CNPs after selection an efficient isolate from twenty isolates (S1-S20) examined the ability to biosynthesis of CNPs based on morphological characters as altering of color, microscopic examination and it was confirmed diagnosis by VITEK 2 Compact System apparatus. The biosynthesis of CNPS were prepared using S2 during an addition of CS to the bacterial culture of free cells at a concentration 2 mg/ml, then centrifugation at 4200 rpm for 15 minutes to separate supernatant that contains NPs from free cells then used for synthesis. The characterization was performed by spectrophotometry of UV, Scanning Electron Microscope (SEM), Energy Dispersive Spectroscopy (EDS). UV-visible spectroscopy analysis was utilized to know CNPs synthesized via absorption at peak 280 nm, analysis by SEM referred to spherical shape with a rough surface of synthesized NPs and characterized with a small size (10-60) nm .The biosynthesized CNPs were analyzed by EDS to estimate elemental percent which was 58.03% for carbon, 36.57% for oxygen, 2.06 % for phosphate, 1.15 % for magnesium, 0.11 % for ferrous, 0.32 % for silicon, 0.47 % for potassium, and 0.76% for sodium. The research showed the antibacterial role of synthesized CNPs when it used different concentrations and it tested against different isolates of S. pneumonia that were collected 120 samples from lung cancer patients and selected as 5, 33, 35, 38, and 61 where gave the greatest inhibition zone on Muller Hinton agar with a diameter 21 mm and the lowest diameter 14 mm as well as our study showed antibiofilm activity of prepared NPs at different concentrations (100, 200, 300, and 400) μg/ml as against all isolates that form biofilms such as isolate 5 and isolate 61, where the greatest inhibition zone with a diameter of 21 mm on Congo red agar.